Table 1

Summary of P. taeda BAC assemblies and whole genome shotgun sequences obtained in this study.

BAC

(Clone)

No. contigs (No. final)

Total length (bp)*

Coverage**

%A

%C

%G

%T


BAC3 (Pt285I20)

9 (1)

142351

6.04× BAC

0.291

0.204

0.195

0.311

BAC12 (Pt314B2)

1 (1)

70964

11.57× BAC

0.322

0.186

0.185

0.307

BAC15 (Pt318P9)

1 (1)

67736

14.38× BAC

0.318

0.182

0.190

0.310

BAC17 (Pt321I16)

3 (1)

88546

8.16× BAC

0.303

0.187

0.188

0.323

BAC19 (Pt331B23)

3 (1)

68919

16.12× BAC

0.315

0.178

0.192

0.315

BAC20 (Pt293K22)

4 (1)

61768

15.78× BAC

0.377

0.185

0.188

0.289

BAC21 (Pt348K5)

3 (1)

93889

8.95× BAC

0.310

0.189

0.190

0.311

BAC31 (Pt737O1)

2 (2)

95786

9.31× BAC

0.319

0.179

0.183

0.318

BAC37 (Pt930E21)

6 (1)

128689

6.68× BAC

0.312

0.193

0.189

0.306

BAC40 (Pt921B18)

4 (1)

104081

10.20× BAC

0.301

0.202

0.183

0.313


40-bp WGS reads

-

3.28E08

0.015× genome

0.304

0.208

0.202

0.287

42-bp WGS reads

-

5.38E08

0.024× genome

0.309

0.194

0.196

0.302

60-bp WGS reads

-

7.98E08

0.036× genome

0.301

0.204

0.201

0.293


*Final length of BAC assembly after vector sequence was removed and linked contigs were joined with N blocks.

**BAC coverage was calculated by dividing the total number of P20 bases by the total amount of pine sequence in each scaffold assembly. Genomic coverage of WGS reads was determined by dividing the total base pairs by the genome size, 2.2E10 bp.

Kovach et al. BMC Genomics 2010 11:420   doi:10.1186/1471-2164-11-420

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