Examples of qRT-PCR validation. The results obtained from the microarray and quantitative RT-PCR follow the same trend, some of which are presented here. The bars (red) indicate the average of five probe set values of candidate exons divided by the average of the corresponding meta probe set values. The lines (blue) represent the fold change of the average of values for the qRT-PCR on the candidate exons as compared to internal controls on the same pre-mRNAs. Embryonic stages represented: E6.5 to E11.5; placental stages: P9.5 to P11.5.
Revil et al. BMC Genomics 2010 11:399 doi:10.1186/1471-2164-11-399