Alternative splicing of Rab6. This pre-mRNA contains two mutually exclusive alternative exons, exons 4 and 5 (see middle panel representing the Rab6 gene structure using the UCSC Genome browser , http://genome.ucsc.edu webcite). The top panel shows raw expression scores for each probe set for the three embryonic developmental stages. There is a slight difference in the expression of the entire transcript, as most probe sets increase their intensities from embryonic day (E) 8.5 to E11.5. This trend is not consistent for exons 4 and 5, as the inclusion of exon 4 actually goes down while the inclusion of exon 5 increases disproportionally. This is further emphasized in the bottom panel, showing the splicing index analysis and the expression values of each exon normalized to adjust for whole transcript expression changes. On the right, the bar graph shows the actual (log2 scale) reduction of exon 4 inclusion across the 3 day time span.
Revil et al. BMC Genomics 2010 11:399 doi:10.1186/1471-2164-11-399