Additional file 6.
Recruitment of TOP mRNAs to polysomes in cardiomyocytes in response to insulin (Microsoft Excel Spreadsheet). Neonatal rat cardiomyocytes were exposed to insulin (50 mU/ml, 1 h) or left unstimulated (controls). Total and polysomal RNA were analysed using microarrays. Probesets corresponding to ribosomal protein mRNAs and other established TOP mRNAs were identified. The mean raw values are provided for controls with the mean fold change (n = 4) induced by insulin relative to controls. *FDR < 0.05 for polysomal RNA from insulin-treated cells vs controls (t-test with Benjamini and Hochberg false discovery rate correction). Mouse and rat 5' UTR sequences up to and including the initiation codon are provided. Sequence identification used FANTOM3 and NCBI databases and the database of transcriptional start sites (DBTSS). The TOP sequence is shown in red. [N.B. For some rat genes, a pseudogene was identified on the NCBI site; the correct gene was identified by comparison with the mouse genome on the basis of intron structure and location relative to adjacent genes (#). For other rat genes, the 5' UTR was short and the genomic sequence was used to identify regions of homology with the mouse genome (†)].
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Markou et al. BMC Genomics 2010 11:343 doi:10.1186/1471-2164-11-343