Table 4

Down-regulated genes after temperature increase up to 37°C (day 4) in L. infantum.

Clone

F

Log2F ± SD

P

GenBank GSS

e-value

Def.

Id.

Annotated Gene Function

qRT-PCR

Fw

Rv

+/-

F ± SD


Lin9E5

-1.77

-0.8 ± 0.3

0.033

GS598994

4e-131

0

b

LinJ35_V3.1150

Oligosaccharyl transferase-like protein

N.D.

Lin40G12

-1.97

-1.0 ± 0.2

0.008

GS598916

2e-161

0

b

LinJ23_V3.1550

Hypothetical protein, unknown function

N.D.

LinJ23_V3.1560

Lathosterol oxidase-like protein

+

5.0 ± 0.7

Lin49B7

-4.38

-2.1 ± 0.0

0.000

GS598995

0

4e-64

a

LinJ34_V3.4160

Phosphatidylinositol-3-kinase (tor2)-like protein

N.D.

Lin 60B1

-2.41

-1.3 ± 0.4

0.025

GS598996

0

4e-162

c

LinJ36_V3.7040

Hypothetical protein, conserved

N.D.

LinJ31_V3.2370

3'-nucleotidase/nuclease, putative

+

7.2 ± 1.0

Lin63F3

-2.23

-1.2 ± 0.4

0.043

GS598997

0

0

a

LinJ36_V3.6550

Glucose transporter lmgt2, putative

+

6.1 ± 0.8

LinJ36_V3.6560

Glucose transporter, putative

+

6.1 ± 0.8

Lin84E8

-2.26

-1.2 ± 0.2

0.007

GS598998

0

0

a

LinJ31_V3.2370

3'-nucleotidase/nuclease, putative

+

7.2 ± 1.0

LinJ31_V3.2380

3'-nucleotidase/nuclease precursor, putative

+

7.2 ± 1.0

Lin85H1

-1.74

-0.8 ± 0.2

0.025

GS598999

1e-57

1e-20

b

LinJ30_V3.3440

CAS/CSE importin domain protein, putative

N.D.

Lin93H3

-2.26

-1.2 ± 04

0.030

GS599000

0

0

a

LinJ31_V3.2370

3'-nucleotidase/nuclease, putative

+

7.2 ± 1.0

LinJ31_V3.2380

3'-nucleotidase/nuclease precursor, putative

+

7.2 ± 1.0

Lin97D1

-3.41

-1.7 ± 0.3

0.001

GS598931

0

0

a

LinJ06_V3.1320

Pteridine transporter, putative

+

2.3 ± 0.3

Lin98G10

-2.13

-1.1 ± 0.2

0.015

GS599001

0

0

b

LinJ30_V3.2770

Hypothetical protein, conserved

N.D.

LinJ30_V3.2780

Superoxide dismutase, putative

+

3.7 ± 0.0

Lin111C2

-2.74

-1.5 ± 0.1

0.002

GS599002

0

0

a

LinJ09_V3.0650

Serine peptidase family S51, peptidase E, putative

N.D.

Lin150E4

-1.86

-0.9 ± 0.3

0.036

GS599003

0

8e-22

b

LinJ13_V3.1060

Calmodulin, putative

N.D.

Lin155H12

-2.34

-1.2 ± 0.3

0.018

GS599004

0

0

a

LinJ36_V3.0250

Peptidyl-prolyl cis-trans isomerase, putative

N.D.

Lin157D8

-2.27

-1.2 ± 0.1

0.002

GS599005

0

-

c

LinJ31_V3.2380

3'-nucleotidase/nuclease precursor, putative

+

7.2 ± 1.0

Lin179B4

-1.76

-0.8 ± 0.1

0.004

GS599006

0

0

b

LinJ07_V3.0030

LinJ07_V3.0040

LinJ07_V3.0050

LinJ07_V3.0060

Hypothetical protein, conserved

Hypothetical protein, conserved

Hypothetical protein, conserved

N.D.

N.D.

N.D.

+

Alpha-adaptin-like protein

5.3 ± 0.4

Lin182F2

-2.23

-1.2 ± 0.1

0.008

GS598952

0

0

b

LinJ25_V3.0740

Eukaryotic initiation factor 5a, putative

N.D.

LinJ25_V3.0750

Eukaryotic initiation factor 5a, putative

N.D.

Lin187C10

-4.72

-2.2 ± 0.4

0.013

GS598953

0

0

b

LinJ06_V3.1320

Pteridine transporter, putative

+

2.3 ± 0.3

Lin204A11

-1.76

-0.8 ± 0.3

0.038

GS599007

-

1e-165

c

LinJ09_V3.0650

Serine peptidase, family S51, peptidase E, putative

N.D.

Lin210B7

-1.74

-0.8 ± 0.2

0.016

GS599008

0

0

a

LinJ32_V3.3690

LinJ32_V3.3700

DEAD/DEAH box helicase, putative

+

N.D.

3.3 ± 0.8

Hypothetical protein, conserved

Lin229E6

-3.30

-1.7 ± 0.3

0.005

GS598963

0

0

a

LinJ06_V3.1320

LinJ06_V3.1330

Pteridine transporter, putative

Coproporphyrinogen III oxidase, putative

+

+

2.3 ± 0.3

4.5 ± 0.6

Lin242E2

-2.37

-1.2 ± 0.4

0.039

GS599009

1e-137

0

a

LinJ31_V3.2370

LinJ31_V3.2380

3'-nucleotidase/nuclease, putative

3'-nucleotidase/nuclease precursor, putative

+

+

7.2 ± 1.0

7.2 ± 1.0

Lin255E12

-2.54

-1.3 ± 0.3

0.011

GS599010

0

0

b

LinJ28_V3.0210

Histone H2B variant

N.D.


This table contains clones that map against down-regulated genes (not hypothetical or unknown) with the single effect of temperature increase (TS). The features described are: clone number; F; base-two logarithmic scale F and SD values; p; GenBank GSS accession numbers; e-values; Def. according to mapping outcomes a, b or c (see brief explanation in the text); Id.; annotated gene function; qRT-PCR. When a given clone overlaps with more than one annotation, stage-specific regulation is only demonstrated if the qRT-PCR result is positive (+). Genes in bold are also down-regulated under TPS.

Alcolea et al. BMC Genomics 2010 11:31   doi:10.1186/1471-2164-11-31

Open Data