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Open Access Methodology article

A simple, high throughput method to locate single copy sequences from Bacterial Artificial Chromosome (BAC) libraries using High Resolution Melt analysis

Giang TH Vu1, Peter DS Caligari23 and Mike J Wilkinson1*

Author Affiliations

1 Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais, Aberystwyth, Ceredigion, SY23 3DA, UK

2 Sumatra Bioscience Pte Ltd, 8 Eu Tong Sen Street, #16-94/95 The Central, 059818, Singapore

3 BioHybrids International Ltd, 11 Penrose Avenue, Woodley, Berkshire RG5 3PA, UK

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BMC Genomics 2010, 11:301  doi:10.1186/1471-2164-11-301

Published: 12 May 2010

Additional files

Additional file 1:

Multiplexing primers. 47 primer pairs mapped to the 1.9 Mb FCA region of A. thaliana [18] were used successfully for pre amplification to demonstrate the high multiplexing capacity.

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