Confidence level of genotyping. f is the probability of amplifying, at least three times, all the different variants of the gene studied for a given sample. This probability depends on n, the total number of sequences per sample, and m, the maximal number of variants for the gene within a sample. T1 is the threshold value that corresponds to the minimal number of sequences required per individual to determine a complete genotype, with a 10-3 probability of missing a variant.
Galan et al. BMC Genomics 2010 11:296 doi:10.1186/1471-2164-11-296