Deciphering the porcine intestinal microRNA transcriptome
1 Freie Universität Berlin, Institute of Veterinary Biochemistry, Oertzenweg 19b, 14163 Berlin, Germany
2 Max Delbrück Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, 13125 Berlin, Germany
3 Max Planck Institute for Molecular Genetics, Department of Human Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany
4 febit biomed gmbh, Im Neuenheimer Feld 519, 69120 Heidelberg, Germany
BMC Genomics 2010, 11:275 doi:10.1186/1471-2164-11-275Published: 30 April 2010
Additional file 1:
Identified porcine miRNAs by miRDeep. Hairpin forming precursors and mature porcine miRNAs were identified from deep sequencing data using the miRDeep algorithm. The table consists of 399 miRDeep scored molecules including assigned porcine miRNAs.
Format: TXT Size: 113KB Download file
Additional file 2:
Identified conserved porcine mature miRNAs by homology search. Mature porcine miRNAs were identified from deep sequencing data by performing a search for conserved miRNAs among the sequencing reads.
Format: TXT Size: 6KB Download file
Additional file 3:
List of validated novel porcine miRNAs by means of customized microarrays or homology search. 201 apparently novel porcine miRNAs out of 399 miRDeep predicted sequences were identified either by means of microarray or by homology search.
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Additional file 4:
miRNAome of the entire porcine intestine identified by means of customized Microarrays. Background subtracted and variance stabilization normalized (VSN) signal intensities of all samples (duodenum, proximal and distal jejunum, ileum, ascending and transverse colon as well as the common reference) is shown as tab delimited text file.
Format: TXT Size: 22KB Download file