nfs expression is dependent on key regulators of the developmental program. Fluorescence detected over the course of development from strains expressing the vector control (black diamonds) or nfs promoted mCherry (PnfsA::mCherry) constructs in the wildtype (black squares) background strains PH1222 and PH1220, respectively (A and B). A. Strains expressing PnfsA::mCherry in the fruA (stars), and csgA (black circles) background strains PH1224 and PH1226, respectively. B. Strains expressing PnfsA::mCherry in the exo (white triangles) and dev (white diamonds) background from strain PH1225 and PH1223, respectively. Strains were induced to develop under submerged culture and fluorescence was recorded by a plate reader at the indicated times.
Müller et al. BMC Genomics 2010 11:264 doi:10.1186/1471-2164-11-264