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Open Access Highly Accessed Research article

Microcollinearity between autopolyploid sugarcane and diploid sorghum genomes

Jianping Wang1, Bruce Roe2, Simone Macmil2, Qingyi Yu3, Jan E Murray1, Haibao Tang4, Cuixia Chen1, Fares Najar2, Graham Wiley2, John Bowers4, Marie-Anne Van Sluys5, Daniel S Rokhsar6, Matthew E Hudson7, Stephen P Moose7, Andrew H Paterson4 and Ray Ming17*

Author Affiliations

1 Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

2 Advanced Center for Genome Technology, Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019-0370, USA

3 AgriLife Research Center, Texas A&M University, Weslaco, TX 78596-8344, USA

4 Plant Genome Mapping Laboratory, University of Georgia, Athens, GA 30602, USA

5 Department of Botany, University of Sao Paulo, 05508-090, Sao Paulo, SP, Brazil

6 Energy Biosciences Institute, University of California, Berkeley, CA 94720, USA

7 Energy Biosciences Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

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BMC Genomics 2010, 11:261  doi:10.1186/1471-2164-11-261

Published: 23 April 2010

Abstract

Background

Sugarcane (Saccharum spp.) has become an increasingly important crop for its leading role in biofuel production. The high sugar content species S. officinarum is an octoploid without known diploid or tetraploid progenitors. Commercial sugarcane cultivars are hybrids between S. officinarum and wild species S. spontaneum with ploidy at ~12×. The complex autopolyploid sugarcane genome has not been characterized at the DNA sequence level.

Results

The microsynteny between sugarcane and sorghum was assessed by comparing 454 pyrosequences of 20 sugarcane bacterial artificial chromosomes (BACs) with sorghum sequences. These 20 BACs were selected by hybridization of 1961 single copy sorghum overgo probes to the sugarcane BAC library with one sugarcane BAC corresponding to each of the 20 sorghum chromosome arms. The genic regions of the sugarcane BACs shared an average of 95.2% sequence identity with sorghum, and the sorghum genome was used as a template to order sequence contigs covering 78.2% of the 20 BAC sequences. About 53.1% of the sugarcane BAC sequences are aligned with sorghum sequence. The unaligned regions contain non-coding and repetitive sequences. Within the aligned sequences, 209 genes were annotated in sugarcane and 202 in sorghum. Seventeen genes appeared to be sugarcane-specific and all validated by sugarcane ESTs, while 12 appeared sorghum-specific but only one validated by sorghum ESTs. Twelve of the 17 sugarcane-specific genes have no match in the non-redundant protein database in GenBank, perhaps encoding proteins for sugarcane-specific processes. The sorghum orthologous regions appeared to have expanded relative to sugarcane, mostly by the increase of retrotransposons.

Conclusions

The sugarcane and sorghum genomes are mostly collinear in the genic regions, and the sorghum genome can be used as a template for assembling much of the genic DNA of the autopolyploid sugarcane genome. The comparable gene density between sugarcane BACs and corresponding sorghum sequences defied the notion that polyploidy species might have faster pace of gene loss due to the redundancy of multiple alleles at each locus.