Table 2

RT-PCR validation of expression values from microarray analysis for selected genes in primary human prostate epithelial cells (hPEC), and LNCaP cells.

RWPE1 - Microarray (n = 4)

hPEC(n = 3)

LNCaP (n = 3)

Gene

6 h

24 h

48 h

#1

#2

#3

8 h


CYP24

1072*

73.9*

63.2*

231 ± 5*

790 ± 192*

959 ± 184*

39.2 ± 2.6*

CD14

226*

27.6*

27.4*

6.3 ± 0.8*

19.6 ± 1.3*

33.8 ± 8.0*

1.6 ± 0.3

TXNRD1

7.1*

1.1

1.5*

2.1 ± 0.2*

6.0 ± 0.1*

9.6 ± 1.8*

4.1 ± 0.9*

IGFBP3

3.0*

-2.7*

-6.4*

3.1 ± 0.4*

38.0 ± 3.1*

22.0 ± 4.8

2.9 ± 0.3*

P2RY2

13.5*

3.8

2.9*

1.6 ± 0.1*

5.2 ± 1.0

4.3 ± 0.2*

1.3 ± 0.1

CYP26B1

5.6*

1.0

1.0

-1.1 ± 0.1

-1.2 ± 0.3

-1.3 ± 0.1

2.7 ± 0.1*

SEMA3B

19.5*

13.4*

18.5*

4.1 ± 0.2*

2.5 ± 0.6

5.7 ± 0.3*

2.2 ± 0.4*

SEMA3F

2.5*

1.5*

1.7*

1.1 ± 0.2

-1.4 ± 0.2

-1.3 ± 0.1

1.8 ± 0.2*

VAV3

-2.7*

-2.3*

-1.4*

-1.3 ± 0.1

-1.2 ± 0.2

-1.3 ± 0.3

1.4 ± 0.1

APCDD1

-5.0*

-2.3*

-2.6*

-3.1 ± 0.2

-1.5 ± 0.3

-1.6 ± 0.3

3.2 ± 0.1*

AKAP12

9.9*

1.0

1.0

4.1 ± 0.8*

5.1 ± 1.8*

5.3 ± 1.9

7.1 ± 1.3

TRPV6

A#

A#

A#

23.3 ± 5.0*

86.0 ± 20.6*

153 ± 40.6*

4.6 ± 0.1*


Data are expressed as mean fold change +SEM of GAPDH-normalized expression (in arbitrary units).

* p < 0.05 (RT-PCR) or FDR<5% (Microarray data), # Absent

Kovalenko et al. BMC Genomics 2010 11:26   doi:10.1186/1471-2164-11-26

Open Data