Genome response to tissue plasminogen activator in experimental ischemic stroke
1 Department of Neurology and M.I.N.D. Institute, University of California at Davis, Sacramento, California 95817, USA
2 Department of Neurology, Cedars-Sinai Medical Center, Los Angeles, California, 90048, USA
BMC Genomics 2010, 11:254 doi:10.1186/1471-2164-11-254Published: 21 April 2010
Tissue plasminogen activator (tPA) is known to have functions beyond fibrinolysis in acute ischemic stroke, such as blood brain barrier disruption. To further delineate tPA functions in the blood, we examined the gene expression profiles induced by tPA in a rat model of ischemic stroke.
tPA differentially expressed 929 genes in the blood of rats (p ≤ 0.05, fold change ≥ |1.2|). Genes identified had functions related to modulation of immune cells. tPA gene expression was found to be dependent on the reperfusion status of cerebral vasculature. The majority of genes regulated by tPA were different from genes regulated by ischemic stroke.
tPA modulates gene expression in the blood of rats involving immune cells in a manner that is dependent on the status of vascular reperfusion. These non-fibrinolytic activities of tPA in the blood serve to better understand tPA-related complications.