Open Access Highly Accessed Research article

Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data

Yukari Nishito1, Yasunori Osana2, Tsuyoshi Hachiya1, Kris Popendorf1, Atsushi Toyoda3, Asao Fujiyama4, Mitsuhiro Itaya5 and Yasubumi Sakakibara1*

Author Affiliations

1 Department of Biosciences and Informatics, Keio University, Hiyoshi, Kohoku-ku, Yokohama, Japan

2 Department of Computer and Information Science, Seikei University, Musashino, Tokyo, Japan

3 Center for Genetic Resource Information, National Institute of Genetics, Shizouka, Japan

4 Principles of Informatics Research Division, National Institute of Informatics, Tokyo, Japan

5 Institute for Advanced Biosciences, Keio University, Minato, Tokyo, Japan

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BMC Genomics 2010, 11:243  doi:10.1186/1471-2164-11-243

Published: 16 April 2010

Additional files

Additional file 1:

Figure S1. Ability of Bacillus subtilis BEST195 to produce Natto by laboratory assay protocol.

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Additional file 2:

Data S1. Re-sequencing results for B. subtilis Marburg 168.

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Additional file 3:

Figure S2. Number of read in plipastatin biosynthesis operon region for both genomes.

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Additional file 4:

Table S1. The list of locations of predicted transposases on BEST195 draft.

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Additional file 5:

Data S2. The list of all the comprehensive sequence alignments for 3610 orthologous genes between B. subtilis natto BEST195 and Marburg 168.

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Additional file 6:

Figure S3. The link plot of five Bacillus species genome comparison, and the dot plot of a pairwise comparison of orthologous genes between BEST195 and Marburg 168, B. amyloliquefaciens, B. licheniformis, and B. pumilus respectively.

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Additional file 7:

Figure S4. Pipeline combining two assembly methods.

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