Research article
A toolkit for rapid gene mapping in the nematode Caenorhabditis briggsae
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* Corresponding author: Bhagwati P Gupta guptab@mcmaster.ca
1 Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110, USA
2 Department of Biology, McMaster University, Hamilton, ON L8S 4K1, Canada
3 Department of Biological Sciences, Wright State University, Dayton, OH 45435, USA
4 Department of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA
5 Department of Biology, University of Maryland, College Park, MD 20742, USA
BMC Genomics 2010, 11:236 doi:10.1186/1471-2164-11-236
Published: 13 April 2010Additional files
Additional file 1:
List of SNPs in C. briggsae. An Excel file containing 23,829 computationally identified SNPs in AF16 and HK104.
Format: XLS Size: 4.9MB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 2:
List of snip-SNPs and RFLP assays. An Excel file containing 1988 SNPs that alter restriction enzyme site (snip-SNPs) along with PCR primers and predicted digestion patterns in AF16 and HK104.
Format: XLS Size: 523KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 3:
List of medium indels and PLP assays. An Excel file containing 214 medium indels along with PCR primers and predicted DNA fragments in AF16 and HK104.
Format: XLS Size: 64KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 4:
List of small indels and PLP assays. The Excel file containing 436 small indels along with PCR primers and predicted DNA fragments in AF16 and HK104.
Format: XLS Size: 133KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 5:
Mapping of unc(sy5506) mutation by single recombinant analysis (SRA). Twenty single F2 mutant animals were individually examined by PCR for the presence of indel bhP26. Four of these (#7, #10, #16, and #17) were found to be recombinants, as judged by the presence of two bands on the agarose gel (corresponding to AF16 and HK104 DNA).
Format: PNG Size: 89KB Download file
Additional file 6:
C. briggsae snip-SNPs identified prior to this work. An Excel file containing nine C. briggsae snip-SNPs that were validated by Hillier et al. [6].
Format: XLS Size: 15KB Download file
This file can be viewed with: Microsoft Excel Viewer