Morphological analysis of Plasmodium falciparum following inhibition of PfSpdSyn with cyclohexylamine. (A) Parasites treated with cyclohexylamine at invasion were arrested in the early trophozoite stage, correlating close to the time of maximal expression of this transcript in the intaerythrocytic developmental cycle (IDC). Untreated parasites matured through mid- into late trophozoites at comparable time points. Arrows indicate the Pearson correlation (r) between samples. In its 48 h lifecycle, the expression of PfSpdSyn is maximally expressed at 18 hours post-invasion (hpi) in a window of expression from 10-40 hpi. (B) Pearson correlations of untreated and treated samples, showing poor correlations of untreated samples to their treated counterparts at later time points (25 and 30 hpi). The approximate time of arrest (between 10 and 18 hpi) is indicated by the highest correlations to the untreated samples at 18 hpi.
Becker et al. BMC Genomics 2010 11:235 doi:10.1186/1471-2164-11-235