Open Access Research article

Plasmodium falciparum spermidine synthase inhibition results in unique perturbation-specific effects observed on transcript, protein and metabolite levels

John VW Becker1, Linda Mtwisha1, Bridget G Crampton1, Stoyan Stoychev1, Anna C van Brummelen1, Shaun Reeksting2, Abraham I Louw2, Lyn-Marie Birkholtz2 and Dalu T Mancama1*

Author Affiliations

1 CSIR Biosciences, PO Box 395, Pretoria, 0001, South Africa

2 Department of Biochemistry, University of Pretoria, Pretoria, Gauteng, 0002, South Africa

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BMC Genomics 2010, 11:235  doi:10.1186/1471-2164-11-235

Published: 12 April 2010

Additional files

Additional file 1:

Table S1. Pearson correlations between individual slides processed in the study. Pearson correlations between technical and biological replicates of slides at each time point in solvent control and drug-treated samples.

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Additional file 2:

Table S2. Differentially expressed up- and downregulated genes at each time point following analysis. PlasmoDB IDs and annotations of differentially expressed and unique up- and downregulated genes at each time point. Overall unique up- and downregulated genes are also listed.

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Additional file 3:

Table S3. Differentially affected proteins at each time point following cyclohexylamine inhibition. Protein response (up- or downregulation), response time point and regulation of the encoding transcripts are indicated. Additionally, protein pI, MW, matched peptides and Protein Pilot scores are shown. Proteins involved in polyamine biosynthesis are indicated in bold.

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