Age-related Decline of Alkaline phosphatase (AP) activity. Osteogenic differentiation involves increased secretion of AP. Alkaline phosphataes was measured in the media of differentiating cell at day 10 as described in Methods. Significantly less AP was secreted from cells taken from mice at age 26 mo (n=4; p<0.01).
Wilson et al. BMC Genomics 2010 11:229 doi:10.1186/1471-2164-11-229