Isolation of Bone Marrow Stem Cells. Bone marrow was aspirated from tibia and fibula of mice (4 per group) at age 2m, 8m, and 26m. 1(A-C) shows cells at the first passage from 2m, 8m, and 26m ages respectively. Morphologies of the cells was heterogenous at this stage. 1(D-F) shows cells after 10 passages again a 2, 8 and 26m respectively, cells at this passage were homogeneous with a flattened morphology.
Wilson et al. BMC Genomics 2010 11:229 doi:10.1186/1471-2164-11-229