BMC Genomics

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Open Access Highly Access Research article

Large-scale analysis of full-length cDNAs from the tomato (Solanum lycopersicum) cultivar Micro-Tom, a reference system for the Solanaceae genomics

Koh Aoki1*, Kentaro Yano2, Ayako Suzuki2, Shingo Kawamura2, Nozomu Sakurai1, Kunihiro Suda1, Atsushi Kurabayashi1, Tatsuya Suzuki3, Taneaki Tsugane3, Manabu Watanabe3, Kazuhide Ooga1, Maiko Torii1, Takanori Narita4, Tadasu Shin-i4, Yuji Kohara4, Naoki Yamamoto2, Hideki Takahashi5, Yuichiro Watanabe6, Mayumi Egusa7, Motoichiro Kodama7, Yuki Ichinose8, Mari Kikuchi9, Sumire Fukushima9, Akiko Okabe9, Tsutomu Arie9, Yuko Sato10, Katsumi Yazawa10, Shinobu Satoh10, Toshikazu Omura11, Hiroshi Ezura11 and Daisuke Shibata1

Author Affiliations

1 Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, 292-0818, Japan

2 Meiji University, 1-1-1 Higashi-mita, Tama-ku, Kawasaki, 214-8571, Japan

3 Chiba Prefectural Agriculture and Forestry Research Center, 808 Daizenno-cho, Midori-ku, Chiba, 266-0006, Japan

4 National Institute of Genetics, Yata 1111, Mishima, 411-8540, Japan

5 Tohoku University, 1-1 Amamiya-machi, Tsutsumidori, Aoba-ku, Sendai, 981-8555, Japan

6 The University of Tokyo, Komaba, Meguro-ku, 153-8902, Japan

7 Tottori University, 4-101 Koyama-minami, Tottori, 680-8553, Japan

8 Okayama University, 1-1-1 Tsushima-naka, Kita-ku, Okayama, 700-8530, Japan

9 Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, 183-8509, Japan

10 Institute of Biological Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8571, Japan

11 Gene Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8571, Japan

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BMC Genomics 2010, 11:210 doi:10.1186/1471-2164-11-210

Published: 30 March 2010

Additional files

Additional file 1:

Tom tissues used for RNA preparation. Detailed description of Micro-Tom tissues used for RNA preparation including tissue positions, treatments, and age of plants when tissues were harvested.

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Additional file 2:

Clone number list. Lists of clone numbers of nrFLcDNAs-sets appeared in the text

Format: XLS Size: 2.1MB Download file

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Additional file 3:

cDNA deived from non-coding RNA. Clone numbers and annotations of nrFLcDNAs that matched to non-coding RNAs registered in NONCODE database.

Format: DOC Size: 27KB Download file

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Additional file 4:

Predicted CDS of 11,597 non-redundant HTCs. A list of amino acid sequences predicted for 11,597 non-redundant HTCs and results of BLASTP search against protein datasets of nr and tomato SBM database http://www.kazusa.or.jp/tomato/ webcite.

Format: XLS Size: 8.2MB Download file

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Additional file 5:

Datasets used for comparative analysis with other plants. Name and version of protein- and tentative consensus-datasets used for comparison of nrFLcDNAs with gene of other plants.

Format: DOC Size: 39KB Download file

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