Variation in the redundancy of sequence reads generated using shotgun libraries prepared with standard and copy-control E. coli strains. A shotgun-subclone library was prepared from each of three BACs [GenBank:AC153092, AC190087, and AC186717] and used to transform either standard DH10B tonA (Std) or copy-control EPI400 (CC) E. coli strains. From each library, paired forward and reverse sequence reads were then generated from randomly selected subclones to produce assemblies that provided an average of eightfold sequence redundancy. Aligned representative regions of the assemblies that highlight differences in sequence redundancy encountered with the two E. coli strains are shown for each BAC. Yellow lines indicate sequence-read redundancies on the upper and lower strands of the indicated sequence contig (black/red line); the horizontal orange lines depict a redundancy value of 10.
Blakesley et al. BMC Genomics 2010 11:21 doi:10.1186/1471-2164-11-21