Open Access Research article

The response to unfolded protein is involved in osmotolerance of Pichia pastoris

Martin Dragosits1, Johannes Stadlmann2, Alexandra Graf13, Brigitte Gasser1, Michael Maurer3, Michael Sauer3, David P Kreil4, Friedrich Altmann2 and Diethard Mattanovich13*

Author Affiliations

1 Department of Biotechnology, BOKU-University of Natural Resources and Applied Life Sciences, Vienna, Austria

2 Department of Chemistry, BOKU-University of Natural Resources and Applied Life Sciences, Vienna, Austria

3 School of Bioengineering, University of Applied Sciences FH-Campus Wien, Vienna, Austria

4 Chair of Bioinformatics, BOKU-University of Natural Resources and Applied Life Sciences, Vienna, Austria

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BMC Genomics 2010, 11:207  doi:10.1186/1471-2164-11-207

Published: 26 March 2010

Additional files

Additional file 1:

Determination of intracellular polyol and trehalose content in P. pastoris upon growth at different osmolarities. contains data on methodology of HPLC measurements, retention times of analytes and analyte concentrations with corresponding standard errors of the mean.

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Additional file 2:

Table of peptides of interesting proteins identified by 2D-DIGE and LC-ESI-MS/MS. contains a list of all peptides assigned to the proteins identified by 2D-DIGE and LC-ESI-MS/MS and described in the manuscript (Table 2). Additionally, scores and scoring schemes are indicated.

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Additional file 3:

Fold-change and one-way ANOVA data for all contrasts of the 2D-DIGE experiment. contains 2D-DIGE data of all comparisons (low to medium, low to high and medium to high) of both strains. Tables contain protein master numbers, short protein names, protein descriptions, fold-changes and corresponding one-way ANOVA values as described for Table 2.

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Additional file 4:

Real-time PCR results of P. pastoris grown at different osmolarities. contains detailed data on real-time PCR. Primers sequences, PCR conditions as well as result diagrams are included.

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Additional file 5:

Quality of microarray experiments and statistical test for osmolarity experiments. contains supplemental data on microarray analysis: Signal intensity plots, correlation of intensities, standard deviations and variations of the microarray experiment. Additionally, results of Hierarchical Cluster Analysis (HCA), Gene Set Analysis (GSA) and Fisher's exact test for the different osmolarities are included.

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Additional file 6:

Microarray results of interesting genes. contains gene expression results of interesting genes, which are described and discussed in the manuscript.

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Additional file 7:

Differences between the non-expressing wt strain and the Fab 3H6 expressing strain of P. pastoris at low osmolarity at the transcript level. contains microarray data on the gene expression differences between the two strains. Spreadsheet 1 contains microarray data for differentially regulated genes (p-value ≤ 0.001). Spreadsheet 2 contains results of gene set analysis (GSA).

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