Table 4

Results of BAC pools screening with molecular markers.

ESTs

SSRs

AFLPs


Markers not assigned to BAC clones*

36

25

13


False positive or not fingerprinted BAC clones

16

7

8


Not amplified in one of the six pools or over-represented in BAC clones

20

18

5


Markers assigned to BAC clones:

138

(4.9 clones*)

108

(3.6 clones*)

149

(2.0 clones*)


Class I: identified two or more BACs assembled in a single contig

82

54

39


Class II: identified only one BAC in a single contig

36

38

69


Class III: identified singletons (unassembled BACs)

6

7

31


Class IV: identified multiple BACs assembled into distinct contigs

14

9

10


Tested

174

133

162


Following BAC screening, each single putatively positive BAC clone has been picked and tested to confirm the primary pool screening result.

* average number of BAC clones identified for each marker type

Scalabrin et al. BMC Genomics 2010 11:204   doi:10.1186/1471-2164-11-204

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