Additional file 1.

BAC-end alignment on the PN40024 sequence to disprove contaminations. contig 71 is apparently highly contaminated as 19 of its 38 clones originated from only 9 plates. To disprove this BAC-end sequencing and corresponding alignment on the PN40024 sequence[32] was carried out. Clones from plate 1070 are contaminated at the library level (they are identical) since corresponding BES align at the same positions. Only two BES hit (with low similarity percentage) on different locations but with their corresponding mate blast hit in the correct location.

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Scalabrin et al. BMC Genomics 2010 11:204   doi:10.1186/1471-2164-11-204