Research article
Physical mapping in highly heterozygous genomes: a physical contig map of the Pinot Noir grapevine cultivar
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* Corresponding author: Michele Morgante michele.morgante@uniud.it
1 Dipartimento di Matematica e Informatica, Università di Udine, Via delle Scienze 206, 33100 Udine, Italy
2 Istituto di Genomica Applicata, Parco Scientifico e Tecnologico di Udine "Luigi Danieli", Via J Linussio 51, 33100 Udine, Italy
3 IASMA Research and Innovation Centre, Foundation Edmund Mach, Via E Mach 1, 38010 San Michele all'Adige (TN), Italy
4 Dipartimento di Scienze Agrarie ed Ambientali, Università degli Studi di Udine, Via delle Scienze 208, 33100 Udine, Italy
5 Keygene NV, PO Box 216, 6700 Wageningen, The Netherlands
6 Dipartimento di Biologia, Università degli Studi di Padova, Via Ugo Bassi 58b, 35121 Padova, Italy
BMC Genomics 2010, 11:204 doi:10.1186/1471-2164-11-204
Published: 26 March 2010Additional files
Additional file 1:
BAC-end alignment on the PN40024 sequence to disprove contaminations. contig 71 is apparently highly contaminated as 19 of its 38 clones originated from only 9 plates. To disprove this BAC-end sequencing and corresponding alignment on the PN40024 sequence[32] was carried out. Clones from plate 1070 are contaminated at the library level (they are identical) since corresponding BES align at the same positions. Only two BES hit (with low similarity percentage) on different locations but with their corresponding mate blast hit in the correct location.
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