Table 3

Quantitative PCR validation of microarray data for select human and mouse genes.

Gene

Reason for Validation

Quantitative PCR P values


Coriell-18

ECACC-6

Nf1-Mouse-12


AUTS2

Overlap of Hs sets

0.0630

0.1790

N/A

C11orf75

Overlap of Hs sets

0.0127

0.2270

N/A

RAB31

Overlap of Hs sets

0.0168

0.0019

N/A

DUSP4

3-Way Overlap of Hs and Mm sets

0.0398

0.1910

N/A

AKAP2

Significant in Coriell-18 (I)

0.1170

0.3540

N/A

CNKSR3

Significant in Coriell-18 (I)

0.0026

0.1850

N/A

IFI6

Significant in Coriell-18 (I)

0.0013

0.5930

N/A

CHURC1

Significant in ECACC-6

0.7390

0.1450

N/A

GSG2

Significant in Nf1-Mm-12*

0.7210

0.1360

N/A

EGLN3

Significant in Coriell-18 (N)

0.0076

0.7630

N/A

MGST3

Significant in Coriell-18 (N)

0.0005

0.6991

N/A

POMC

Significant in Coriell-18 (N)

0.0170

0.5582

N/A

Aytl2

Overlap of Hs-Mm sets

N/A

N/A

0.1900

Crip1

Overlap of Hs-Mm sets

N/A

N/A

0.5830

Pecr

Overlap of Hs-Mm sets

N/A

N/A

0.5840

Zfp36l1

Overlap of Hs-Mm sets

N/A

N/A

0.5140

Dusp4

3-Way Overlap of Hs and Mm sets

N/A

N/A

0.7410

Cxcr3

Significant in Nf1-Mm-12*

N/A

N/A

0.0052

Gsg2

Significant in Nf1-Mm-12*

N/A

N/A

0.0262

Igtp

Significant in Nf1-Mm-12*

N/A

N/A

0.0380

Inpp5k

Significant in Nf1-Mm-12

N/A

N/A

0.0720

Rnf43

Significant in Nf1-Mm-12*

N/A

N/A

0.0004


Statistical significance of differential expression of genes was evaluated by t test. Genes with nominal P values < 0.05 were considered significant and are shown in boldface. "(I)" and "(N)" in "Significant in Coriell-18 (I)/(N)" indicate that sample set Coriell-18 was analyzed on the Illumina or NHGRI platform, respectively. "Hs" designates human (Coriell-18 and ECACC-6) datasets; "Mm" designates murine (Nf1-Mouse-12) dataset. * - nominal P value < 0.001. "N/A" - not applicable.

Pemov et al. BMC Genomics 2010 11:194   doi:10.1186/1471-2164-11-194

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