Open Access Research article

Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome

Ralph Kissen1, Per Winge1, Diem Hong Thi Tran1, Tommy S Jørstad13, Trond R Størseth2, Tone Christensen14 and Atle M Bones1*

Author Affiliations

1 Department of Biology, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway

2 SINTEF Fisheries and Aquaculture, NO-7465 Trondheim, Norway

3 Current address: Scandpower AS, NO-7462 Trondheim, Norway

4 Current address: Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology (NTNU), NO-7489 Trondheim, Norway

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BMC Genomics 2010, 11:190  doi:10.1186/1471-2164-11-190

Published: 22 March 2010

Additional files

Additional file 1:

Transcriptional data of glu1-2. Detailed lists of genes whose expression is affected in leaves and roots of 18 day old glu1-2 mutant plants

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Additional file 2:

GOstat analysis of glu1-2 transcriptional data. GO_biological_process terms identified by GOstat [13] as being overrepresented (P = 0.01 with FDR/Benjamini correction) among the genes affected in glu1-2 mutant leaves and roots.

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Additional file 3:

PathExpress analysis of glu1-2 transcriptional data. Identification by PathExpress [14] of affected pathways among the sets of genes whose expression is affected in the glu1-2 mutant.

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Additional file 4:

GeneBins analysis of glu1-2 transcriptional data. BINs identified by GeneBins [15] as being affected in the datasets of genes that show a different expression in glu1-2 organs compared to wild type.

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Additional file 5:

2-oxoglutarate synthesis and TCA cycle. Genes whose expression is affected in glu1-2 mutant leaves and that encode enzymes involved in 2-oxoglutarate synthesis and the TCA cycle.

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Additional file 6:

Photosynthesis and related pathways. Genes whose expression is affected in glu1-2 mutant leaves and roots and that encode enzymes involved in photosynthesis related GO categories and bins as identified by GOstat [13], AmiGO [132] and MapMan [12] respectively.

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Additional file 7:

Transcription factors. Genes encoding WRKY, C2H2, CCCH, MYB, bHLH or AP2/ERF transcription factors and that show different expression in the glu1-2 mutant.

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Additional file 8:

Shikimate pathway and flavonoid biosynthesis. Genes implicated in the shikimate pathway and flavonoid biosynthesis, and that show different expression in the glu1-2 mutant.

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Additional file 9:

ABC transporters. Genes whose expression is affected in the glu1-2 mutant and that encode ABC transporters.

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Additional file 10:

Cytochrome P450 monooxygenases. Cytochrome P450-encoding genes whose expression is affected in the glu1-2 mutant.

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Additional file 11:

Glutathione S-transferases. Genes encoding glutathione S-transferases (GSTs) and that show different expression in the glu1-2 mutant.

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Additional file 12:

UDP-glycosyltransferases. Genes encoding UDP-glycosyltransferases (UGTs) and that show different expression in the glu1-2 mutant.

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Additional file 13:

MATE efflux family proteins. Genes coding for multidrug and toxic compound extrusion (MATE) efflux carriers and that show different expression in the glu1-2 mutant.

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Additional file 14:

Comparison with catalase 2 deficient plants. Comparison of induced transcriptional responses in catalase 2 deficient plants CAT2HP1 and glu1-2 mutant leaves.

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