Confirmation of alternative splicing of LRR-RLKs by RT-PCR. Total RNA was extracted from inflorescence and leaf of Arabidopsis, respectively. The reverse transcribed single-stranded cDNA was used as template for nested PCR with variant-specific primers to confirm potentially alternatively spliced variants emerged from the predicted mRNA sequences and previous reports. The nested PCR products were separated on a 1.5% (w/v) agarose gel. The AGI numbers for examined genes are shown on the top of the lanes. For each gene, the left lane shows the result from inflorescence and the right lane shows the result from leaf. a, Previously reported variant [GenBank:BT011697] of At1g56140; b, Predicted sequence [GenBank:NM_104492] of At1g56140; c, Predicted sequence [GenBank:NM_125922] of At5g65240; d, Previously reported variant [GenBank:AY059844] of At5g65240. M, molecular weight markers (kb).
Gou et al. BMC Genomics 2010 11:19 doi:10.1186/1471-2164-11-19