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Open Access Highly Accessed Research article

Transcriptome sequencing in an ecologically important tree species: assembly, annotation, and marker discovery

Thomas L Parchman1*, Katherine S Geist2, Johan A Grahnen3, Craig W Benkman4 and C Alex Buerkle1

Author Affiliations

1 Department of Botany, University of Wyoming, Laramie, WY 82071, USA

2 Department of Biology, Beloit College, Beloit, WI 53511, USA

3 Department of Molecular Biology, Laramie, WY 82071, USA

4 Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA

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BMC Genomics 2010, 11:180  doi:10.1186/1471-2164-11-180

Published: 16 March 2010

Additional files

Additional file 1:

Supplementary File S1 -- Primer sequences for SSR loci. Information for SSR primers designed in BatchPrimer3 using stringent criteria. Provided for each locus is information on the sequence ID, location of SSR, location of both primers, primer sequences, length of primer sequences, melting temps, GC%, SSR motif, motif length, SSR sequence, and SSR length.

Format: TXT Size: 770KB Download file

Open Data