Table 5 |
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|
The cytosolic proteome of H. mustelae determined by LC-MS |
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|
Ranka |
Locus |
Annotation |
Mw Da |
MOWSE Scoreb |
Coveragec% |
emPAId |
Mol% |
|
|
|||||||
|
1 |
HMU14210 |
fldA flavodoxin 1 |
18,147 |
620 |
56 |
6.74 |
7.35 |
|
2 |
HMU05200 |
thiJ 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis protein |
20,365 |
212 |
32 |
3.64 |
3.97 |
|
3 |
HMU01210 |
tpx probable thiol peroxidase |
17,993 |
886 |
62 |
3.43 |
3.75 |
|
4 |
HMU12840 |
aroQ 3-dehydroquinate dehydratase |
17,477 |
161 |
21 |
2.98 |
3.25 |
|
5 |
HMU12860 |
sodB superoxide dismutase (Fe) |
24,576 |
534 |
45 |
2.41 |
2.63 |
|
6 |
HMU00320 |
undefined product |
8,942 |
162 |
54 |
2.16 |
2.36 |
|
7 |
HMU05470 |
acpP acyl carrier protein |
8,469 |
139 |
48 |
2.16 |
2.36 |
|
8 |
HMU03120 |
tuf elongation factor TU |
43,570 |
1695 |
62 |
2.09 |
2.28 |
|
9 |
HMU12690 |
possible bacterioferritin |
17,198 |
562 |
46 |
1.99 |
2.17 |
|
10 |
HMU09770 |
trxA thioredoxin |
11,553 |
361 |
36 |
1.89 |
2.06 |
|
11 |
HMU04180 |
hypothetical protein Cj1613c |
30,321 |
755 |
29 |
1.71 |
1.87 |
|
12 |
HMU03320 |
ahpC alkyl hydroperoxide reductase |
22,088 |
572 |
44 |
1.68 |
1.83 |
|
13 |
HMU14370 |
cft ferritin |
19,056 |
276 |
45 |
1.42 |
1.55 |
|
14 |
HMU00950 |
rpsA 30S ribosomal protein S1 |
60,196 |
208 |
7 |
1.31 |
1.43 |
|
15 |
HMU10800 |
lpsJ succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B |
23,051 |
501 |
38 |
1.15 |
1.26 |
|
16 |
HMU04000 |
groEL 60 kD chaperonin (cpn60) |
57,443 |
1474 |
48 |
1.02 |
1.11 |
|
17 |
HMU03150 |
rplK 50S ribosomal protein L11 |
12,586 |
473 |
48 |
0.90 |
0.99 |
|
18 |
HMU01260 |
ald alanine dehydrogenase |
39,844 |
662 |
32 |
0.82 |
0.9 |
|
19 |
HMU07380 |
hupB DNA-binding protein HU homolog |
10,122 |
352 |
56 |
0.81 |
0.89 |
|
20 |
HMU10120 |
peb4[1]f2 major antigenic peptide PEB3ll binding factor 2 |
31,816 |
750 |
39 |
0.77 |
0.85 |
|
21 |
HMU07050 |
rplI 50S ribosomal protein L9 |
16,251 |
460 |
49 |
0.74 |
0.81 |
|
22 |
HMU04730 |
oorB OORB subunit of 2-oxoglutarate:acceptor oxidoreductase |
30,613 |
423 |
28 |
0.74 |
0.81 |
|
23 |
HMU14090 |
putative thioredoxin |
11,796 |
267 |
39 |
0.730 |
0.8 |
|
24 |
HMU04740 |
oorA OORA subunit of 2-oxoglutarate:acceptor oxidoreductase |
40,773 |
554 |
36 |
0.70 |
0.77 |
|
25 |
HMU02170 |
cheY chemotaxis regulatory protein |
13,894 |
208 |
25 |
0.70 |
0.76 |
|
26 |
HMU11060 |
putative putative aminotransferase (nifS protein homolog) |
43,365 |
424 |
26 |
0.66 |
0.73 |
|
27 |
HMU00290 |
fbp putative putative fructose-1,6-bisphosphatase |
30,550 |
323 |
20 |
0.66 |
0.73 |
|
28 |
HMU03180 |
rplL 50S ribosomal protein L7/L12 |
12,946 |
259 |
44 |
0.66 |
0.73 |
|
29 |
HMU00100 |
putative putative acyl-CoA thioester hydrolase |
16,960 |
168 |
20 |
0.63 |
0.7 |
|
30 |
HMU13330 |
undefined product |
8,555 |
166 |
49 |
0.63 |
0.7 |
|
31 |
HMU03850 |
flaG possible flagellar protein |
14,814 |
452 |
34 |
0.62 |
0.69 |
|
32 |
HMU03060 |
ureB urease alpha subunit |
61,146 |
743 |
26 |
0.62 |
0.68 |
|
33 |
HMU09760 |
trxB thioredoxin reductase |
33,972 |
587 |
35 |
0.62 |
0.68 |
|
34 |
HMU07390 |
ndk nucleoside diphosphate kinase |
15,293 |
291 |
25 |
0.61 |
0.67 |
|
35 |
HMU05040 |
putative hydantoin utilization protein B |
83,272 |
948 |
28 |
0.60 |
0.66 |
|
36 |
HMU10790 |
scoA succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A |
25,076 |
305 |
32 |
0.58 |
0.64 |
|
37 |
HMU03990 |
groES 10 kD chaperonin (cpn10) |
10,185 |
129 |
34 |
0.58 |
0.64 |
|
38 |
HMU13450 |
hypothetical protein Cj0706 |
27,047 |
694 |
49 |
0.55 |
0.61 |
|
39 |
HMU10960 |
katA catalase |
52,490 |
781 |
39 |
0.53 |
0.59 |
|
40 |
HMU03670 |
aspA aspartate ammonia-lyase |
51,394 |
620 |
31 |
0.51 |
0.57 |
|
41 |
HMU10290 |
rpoA DNA-directed RNA polymerase alpha chain |
37,551 |
308 |
25 |
0.50 |
0.55 |
|
42 |
HMU11440 |
putative putative nucleotide phosphoribosyltransferase |
17,378 |
164 |
25 |
0.50 |
0.55 |
|
43 |
HMU10630 |
putative putative periplasmic protein |
21,100 |
282 |
24 |
0.48 |
0.53 |
|
44 |
HMU05030 |
putative putative hydantoinase A |
78,444 |
681 |
24 |
0.47 |
0.52 |
|
45 |
HMU11200 |
glyA serine hydroxymethyltransferase |
45,885 |
461 |
24 |
0.48 |
0.52 |
|
46 |
HMU08860 |
hypB hydrogenase isoenzymes formation protein |
26,838 |
328 |
28 |
0.46 |
0.51 |
|
47 |
HMU01430 |
putative putative periplasmic cytochrome C |
10,944 |
178 |
36 |
0.46 |
0.51 |
|
48 |
HMU06880 |
yabJ putative putative regulatory protein |
13,506 |
163 |
26 |
0.46 |
0.51 |
|
49 |
HMU09820 |
surE SurE protein homolog |
28,918 |
352 |
25 |
0.44 |
0.49 |
|
50 |
HMU12070 |
putative putative exported protein |
16,551 |
270 |
33 |
0.44 |
0.49 |
|
|
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|
The 50 most abundant proteins are tabulated. a. Relative abundance ranked by mol% b. Score for the entire protein derived by MASCOT, and made up of the individual scores given to each peptide sequence c. Proportion of each protein sequence identified d. Exponentially modified protein abundance index. See Methods for details and reference. |
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|
O'Toole et al. BMC Genomics 2010 11:164 doi:10.1186/1471-2164-11-164 |
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