Table 5

The cytosolic proteome of H. mustelae determined by LC-MS

Ranka

Locus

Annotation

Mw Da

MOWSE Scoreb

Coveragec%

emPAId

Mol%


1

HMU14210

fldA flavodoxin 1

18,147

620

56

6.74

7.35

2

HMU05200

thiJ 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis protein

20,365

212

32

3.64

3.97

3

HMU01210

tpx probable thiol peroxidase

17,993

886

62

3.43

3.75

4

HMU12840

aroQ 3-dehydroquinate dehydratase

17,477

161

21

2.98

3.25

5

HMU12860

sodB superoxide dismutase (Fe)

24,576

534

45

2.41

2.63

6

HMU00320

undefined product

8,942

162

54

2.16

2.36

7

HMU05470

acpP acyl carrier protein

8,469

139

48

2.16

2.36

8

HMU03120

tuf elongation factor TU

43,570

1695

62

2.09

2.28

9

HMU12690

possible bacterioferritin

17,198

562

46

1.99

2.17

10

HMU09770

trxA thioredoxin

11,553

361

36

1.89

2.06

11

HMU04180

hypothetical protein Cj1613c

30,321

755

29

1.71

1.87

12

HMU03320

ahpC alkyl hydroperoxide reductase

22,088

572

44

1.68

1.83

13

HMU14370

cft ferritin

19,056

276

45

1.42

1.55

14

HMU00950

rpsA 30S ribosomal protein S1

60,196

208

7

1.31

1.43

15

HMU10800

lpsJ succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B

23,051

501

38

1.15

1.26

16

HMU04000

groEL 60 kD chaperonin (cpn60)

57,443

1474

48

1.02

1.11

17

HMU03150

rplK 50S ribosomal protein L11

12,586

473

48

0.90

0.99

18

HMU01260

ald alanine dehydrogenase

39,844

662

32

0.82

0.9

19

HMU07380

hupB DNA-binding protein HU homolog

10,122

352

56

0.81

0.89

20

HMU10120

peb4[1]f2 major antigenic peptide PEB3ll binding factor 2

31,816

750

39

0.77

0.85

21

HMU07050

rplI 50S ribosomal protein L9

16,251

460

49

0.74

0.81

22

HMU04730

oorB OORB subunit of 2-oxoglutarate:acceptor oxidoreductase

30,613

423

28

0.74

0.81

23

HMU14090

putative thioredoxin

11,796

267

39

0.730

0.8

24

HMU04740

oorA OORA subunit of 2-oxoglutarate:acceptor oxidoreductase

40,773

554

36

0.70

0.77

25

HMU02170

cheY chemotaxis regulatory protein

13,894

208

25

0.70

0.76

26

HMU11060

putative putative aminotransferase (nifS protein homolog)

43,365

424

26

0.66

0.73

27

HMU00290

fbp putative putative fructose-1,6-bisphosphatase

30,550

323

20

0.66

0.73

28

HMU03180

rplL 50S ribosomal protein L7/L12

12,946

259

44

0.66

0.73

29

HMU00100

putative putative acyl-CoA thioester hydrolase

16,960

168

20

0.63

0.7

30

HMU13330

undefined product

8,555

166

49

0.63

0.7

31

HMU03850

flaG possible flagellar protein

14,814

452

34

0.62

0.69

32

HMU03060

ureB urease alpha subunit

61,146

743

26

0.62

0.68

33

HMU09760

trxB thioredoxin reductase

33,972

587

35

0.62

0.68

34

HMU07390

ndk nucleoside diphosphate kinase

15,293

291

25

0.61

0.67

35

HMU05040

putative hydantoin utilization protein B

83,272

948

28

0.60

0.66

36

HMU10790

scoA succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A

25,076

305

32

0.58

0.64

37

HMU03990

groES 10 kD chaperonin (cpn10)

10,185

129

34

0.58

0.64

38

HMU13450

hypothetical protein Cj0706

27,047

694

49

0.55

0.61

39

HMU10960

katA catalase

52,490

781

39

0.53

0.59

40

HMU03670

aspA aspartate ammonia-lyase

51,394

620

31

0.51

0.57

41

HMU10290

rpoA DNA-directed RNA polymerase alpha chain

37,551

308

25

0.50

0.55

42

HMU11440

putative putative nucleotide phosphoribosyltransferase

17,378

164

25

0.50

0.55

43

HMU10630

putative putative periplasmic protein

21,100

282

24

0.48

0.53

44

HMU05030

putative putative hydantoinase A

78,444

681

24

0.47

0.52

45

HMU11200

glyA serine hydroxymethyltransferase

45,885

461

24

0.48

0.52

46

HMU08860

hypB hydrogenase isoenzymes formation protein

26,838

328

28

0.46

0.51

47

HMU01430

putative putative periplasmic cytochrome C

10,944

178

36

0.46

0.51

48

HMU06880

yabJ putative putative regulatory protein

13,506

163

26

0.46

0.51

49

HMU09820

surE SurE protein homolog

28,918

352

25

0.44

0.49

50

HMU12070

putative putative exported protein

16,551

270

33

0.44

0.49


The 50 most abundant proteins are tabulated.

a. Relative abundance ranked by mol%

b. Score for the entire protein derived by MASCOT, and made up of the individual scores given to each peptide sequence

c. Proportion of each protein sequence identified

d. Exponentially modified protein abundance index. See Methods for details and reference.

O'Toole et al. BMC Genomics 2010 11:164   doi:10.1186/1471-2164-11-164

Open Data