Sequence analysis and map position of candidate gene StTLRP associated with potato tuber cooking type. Alignment of cDNA sequences representing the identified allelic variation of the TLRP gene and the oligo sequence (micro.187.c2) present on the microarray (a). Alignment of the predicted protein sequences of the potato TLRP allele variants and the tomato homolog (CAA54561). The signal peptide and the Cys-binding domain are indicated (b). Marker development based on the variation in length of PCR products revealing the allelic variation found between both parental clones and the presence of deletion site in the unique E-allele (TLRP_Δ7) (indicated with arrow lane 1). Allele-specific amplification of the TLRP_Δ7 allele in the E-parent (lane 3) (c). Map position of the potato TLRP gene on the long arm of chromosome 9 under the QTL for cooking type analyzed for two years (1, 2) (d).
Kloosterman et al. BMC Genomics 2010 11:158 doi:10.1186/1471-2164-11-158