Open Access Methodology article

Transcribed-ultra conserved region expression profiling from low-input total RNA

Paola Scaruffi1*, Sara Stigliani1, Simona Coco1, Franscesca Valdora2, Carla De Vecchi1, Stefano Bonassi3 and Gian Paolo Tonini1

Author Affiliations

1 Translational Paediatric Oncology, National Cancer Research Institute (IST), Largo R Benzi 10, Genoa, 16132, Italy

2 Department of Oncology and Genetics (DOBIG), University of Genoa, Largo R Benzi 10, Genoa, 16132, Italy

3 Clinical and Molecular Epidemiology, IRCCS San Raffaele Pisana, Via della Pisana 235, Roma, 00163, Italy

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BMC Genomics 2010, 11:149  doi:10.1186/1471-2164-11-149

Published: 3 March 2010

Additional files

Additional file 1:

Raw Cq data. Raw Cq values for each qPCR reaction, according to the Real-time PCR Data Markup Language (RDML).

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Additional file 2:

Tm data. Melting temperature values for each qPCR reaction.

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Additional file 3:

MIQE checklist. Checklist according to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments.

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Additional file 4:

Figure S1. Plots showing the correlation between the average Cq value and the standard deviation for T-UCRs in GI-ME-N cell line, using reverse transcriptase of total RNA (A) and amplification system (B). Bar plots display the mean SD value for T-UCRs with an average Cq value ranging between 15-20, >20-25, >25-30, and >30-35 cycles.

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