Validation of the NaCl time-course by RT-PCR. The mRNA levels of 6 strongly up-regulated genes, N-myc, c-fos, Dusp4, Tpp Akr1b3 (AR) and Hspa4l, were determined by RT-PCR and corrected by the mRNA levels of GAPDH, quantified and plotted (right panels) alongside with the mRNA levels obtained from the microarrays (left panels). Black circles indicate wt MEFs and grey circles wt MEFs pre-treated with SB203580. The average of two independent experiments is shown.
Ferreiro et al. BMC Genomics 2010 11:144 doi:10.1186/1471-2164-11-144