Validation of the microarray data by RT-PCR. The mRNA levels of 4 strongly up-regulated genes, A20, Ptgs2/Cox2, Btg2 and c-Jun, were determined by RT-PCR, corrected by the mRNA levels of GAPDH, quantified and plotted (right panels) alongside with the mRNA levels obtained from the microarrays (left panels). Black bars represent wt MEFs, blue bars p38α-/- knock out MEFs and red bars wt MEFs pre-treated with SB203580. The average of two independent experiments is shown.
Ferreiro et al. BMC Genomics 2010 11:144 doi:10.1186/1471-2164-11-144