Amplification of known putative genes using random selected BAC clone samples from different genotypes. All of BAC clones used in the BAC genotyping were selected based on the hybridization pattern against F, M and R probes that correspond to 5'-end (F), middle (M) and 3'-end (R) of the Mj024A04-sequence as described in the result and method. All genotypes were classified based on 3 distinct microsatellite repeats as described in the result. The putative genes (indicated in top line) were detected with BAC clones that showed different genotypes (indicated in left column). Reactions without templates (Nt) and primers (Np) are included as negative control. Reactions with Mj024A04 as template are also included as positive control.
Koyama et al. BMC Genomics 2010 11:141 doi:10.1186/1471-2164-11-141