BAC-FISH of selected BAC clones with the T. aestivum mitotic metaphase chromosomes. (A) Clone 3AS0096P02 (labeled pale orange) showed a dispersed hybridization across all 21 pairs of chromosomes and could not be localized to a genome. (B) Clone 3AS0034G08 (labeled green) hybridized with 7 pairs of chromosomes belonging to the D genome identified using a probe for the Afa repeat family (labeled red). (C and D) Clones 3DS0002N13 (C) and 3DS0019H15 (D) (both labeled green) showed dispersed signal on 7 pairs of chromosomes belonging to the A genome, which were identified by the failure to hybridize with GAA microsatellites (labeled yellow) and an Afa family repeat (labeled red). All chromosomes were counterstained with DAPI (blue).
Luo et al. BMC Genomics 2010 11:122 doi:10.1186/1471-2164-11-122