Table 2

Identification of nine selected nitrogen fixation-inducible genes and nitrogenase activity of the corresponding mutant strains

Gene ID

Functional description

Involvement in nitrogen fixation in other systems

Nitrogenase activitya, %


PST0446

Cytoplasmic membrane protein

Unknown

64

PST0874

Chemotactic transducer

Unknown

52

PST1325

conserved hypothetical protein

Unknown

23

PST1521

outer membrane protein

Unknown

68

PST2508

methyl-accepting chemotaxis transducer

Unknown

92

PST3417

predicted transcriptional regulators

Unknown

58

PST3422

outer membrane protein

Unknown

96

PST3621

transcriptional regulator, AraC family

Unknown

80

PST4084

ABC-type amino acid transport/signal transduction systems

Unknown

54


aNitrogenase activity of nine non-polar mutants (enabling transcription of downstream genes) is expressed as % of wild type A1501 activity, which is in the range of 1645 ± 169 nmol ethylene/min/mg protein. Nitrogenase activity was examined under nitrogen fixation conditions (0.1 mM ammonium and 0.5% oxygen tension). Data are expressed as the means of three independent experiments.

Yan et al. BMC Genomics 2010 11:11   doi:10.1186/1471-2164-11-11

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