Figure 6.

Phenotypic analyses of the mutation inserted into the pnfA gene. (A) Quantitative RT-PCR analysis of pnfA expression in the wild type A1501, nifA mutant A1506, and rpoN mutant A1550 strains under nitrogen fixation conditions. (B) Quantitative RT-PCR analysis of nifH expression in the wild type A1501 and mutant A1325 strains under nitrogen fixation conditions. (C) The effect of different oxygen concentrations on nitrogenase activity in the wild type A1501 and mutant A1325 strains. (D) Nitrogenase activity in the wild type A1501 and mutant A1325 strains grown under anaerobic conditions with or without 1 mM nitrate. * The nitrogenase activity of the mutant strain is expressed as a percentage (%) of the wild type A1501 activity. Results shown are representative of four (C) or three (D) independent experiments.

Yan et al. BMC Genomics 2010 11:11   doi:10.1186/1471-2164-11-11
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