Table 1 |
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The designed primers for CYP2D6 analysis obtained from RExPrimer |
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Name |
Sequence 5' → 3' |
Purpose |
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CYP2D6_1F |
GGCCTACCCTGGGTAAGGGCCTGGAGCAGGA |
whole gene amplification |
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CYP2D6_1R |
CTCAGCCTCAACGTACCCCTGTCTCAAATGCG |
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|
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CYP2D6_2F |
GAGACTCCTCGGTCTCTCG |
penta-plex PCR |
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CYP2D6_2R |
TAATGCCTTCATGGCCACGCG |
|
|
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CYP2D6_3F |
AGGCCTTCCTGGCAGAGATGAAG |
penta-plex PCR |
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CYP2D6_3R |
CCCCTGCACTGTTTCCCAGA |
|
|
|
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CYP2D6_4F |
CCAGCCACCATGGTGTCTTTG |
penta-plex PCR |
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CYP2D6_4R |
GCCTCAACGTACCCCTGTCTC |
|
|
|
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DMD_F |
TTGTCGGTCTCCTGCTGGTCAGTG |
one-copy internal standard in penta-plex PCR |
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DMD_R |
CAAAGCCCTCACTCAAACATGAAGC |
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|
|
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LDL_F |
TACAAGTGCCAGTGTGAGGAAG |
two-copy internal standard in penta-plex PCR |
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LDL_R |
GTGCAAAGTTCAGAGGATGAAACT |
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|
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Rows 2-6 represent semiquantitative analysis of CNV |
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Piriyapongsa et al. BMC Genomics 2009 10(Suppl 3):S4 doi:10.1186/1471-2164-10-S3-S4 |
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