Table 3

Sequence characteristics that consistently show either positive or negative impacts on shRNA efficacy in Least Square Analysis.

Positive Parameters


Parameter

Position

Coefficient


G

3

8.01

G

5

9.11

A

11

7.59

T

14

6.16

CA

6–7

11.48

CC

8–9

11.63

TG

10–11

19.58

GA

13–14

31.32

TA

17–18

6.36

TA

18–19

24.11

Negative Parameters

Parameter

Position

Coefficient

TA

4–5

-35.04

TA

6–7

-23.35

GG

8–9

-21.00

GC

9–10

-24.66

GA

17–18

-15.08


Zhou and Zeng BMC Genomics 2009 10(Suppl 1):S9   doi:10.1186/1471-2164-10-S1-S9

Open Data