Figure 3.

Quantitative Real-time PCR analysis of gene expression in Plectus murrayi in response to desiccation. Values were determined using qRT-PCR and represents relative expression of genes from desiccated to undesiccated nematodes (control). The relative expression of the target gene (Pm-alh: aldehyde dehydrogenase; Pm-tps: trehalose-6-phosphate synthase; Pm-gpx: glutathione peroxidise; Pm-afp: antifreeze protein; Pm-hsp-70: heat shock protein 70; Pm-lea: late embryogenesis abundant protein; Pm-gk: glycerol kinase; Pm-ms: malate synthase; Pm-gsy: glycogen synthase; Pm-hsp-90: heat shock protein 90; Pm-rpl-4: ribosomal protein-4; Pm-desc-1: novel protein I; Pm-desc-2: novel protein II; Pm-gst-1: glutathione s-transferase 1,) normalized to Pm-18s:18S rRNA and relative to the expression of control, was calculated for each sample using the 2^-ΔΔCT method [87]. Gene expression was determined in each sample using three independent technical replicates. A transcript with relative abundance of one is equivalent to the abundance of 18S rRNA. Bars represent standard errors calculated from three replicates of each experiment. *Significant difference (P < 0.05) from control.