Cross species analysis of canine and human osteosarcoma are not distinguishable by global gene expression signature. Comparative genomic analysis was performed by defining the differentially expressed genes between osteosarcoma and normal tissues (adjusted p value < 0.01) and by establishing orthologues between species. After Entrez Gene ID alignment, 265 genes were used to cluster the human and canine osteosarcomas, normal tissues and cell lines. Hierarchical clustering resulted in complete branching of normal and tumor samples, and normal organs could be further defined based on species of origin. Among the 30 primary tumor samples, branching of human and canine osteosarcoma is not divided by species. This suggests that similarities in gene expression signatures in osteosarcoma are due to shared biology across species.
Paoloni et al. BMC Genomics 2009 10:625 doi:10.1186/1471-2164-10-625