Table 3

The effect of repeat unit sequence on microsatellite heterozygosity for tetra-nucleotide loci

AAAT & TTTA

AATG

CATA & GTAT

TTCA

GATG

ATCT & TAGA

AAGG & TTCC

Number of loci

30

4

8

3

5

253

18


Mean He

0.683

0.691

0.714

0.719

0.722

0.744

0.789


AAAT [AATA-ATAA-TAAA] & TTTA [TTAT-TATT-ATTT]

0.979

0.407

0.416

0.421

4.49 × 10-4

4.80 × 10-5

AATG [ATGA-TGAA-GAAT]

0.808

0.857

0.556

0.229

0.066

CATA [ATAC-TACA-ACAT] & GTAT [TATG-ATGT-TGTA]

0.921

1

0.239

0.011

TTCA [TCAT-CATT-ATTC]

0.786

0.541

0.080

GATG [ATGG-TGGA-GGAT]

0.304

0.019

ATCT [TCTA-CTAT-TATC] & TAGA [AGAT-GATA-ATAG]

0.003

AAGG [AGGA-GGAA-GAAG] & TTCC [TCCT-CCTT-CTTC]


P values are shown for two-sided Wilcoxon rank-sum tests for differences in heterozygosity (He) between the different repeat unit sequences of microsatellites with one tetra-nucleotide STR region. Four loci were excluded from these comparisons because their repeat unit sequences only appeared once (TTTG and GAAA) or twice (TCCA) in the data set. Tests with P < 0.05 are highlighted in bold.

Pemberton et al. BMC Genomics 2009 10:612   doi:10.1186/1471-2164-10-612

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