Table 1

Classification of differential gene expression of esfenvalerate responding genes in 52-d old H. transpacificus following 24 h exposure as identified by microarray analyses.

BLASTX top hit

Species match

Accession No.

Score

E-value

Concentration

Fold Change (+/-)

P-VALUE (or cut-off)


    Neuromuscular

titin a

Danio rerio

ABG48500

110

4.00E-23

0.0625

1.3277

0.0625

**

smooth muscle cell-specific protein SM22 alpha

Epinephelus coioides

ABW04145

349

1.00E-94

0.1250

1.2252

0.0029

toxin-1

Oncorhynchus mykiss

AAM21198

116

5.00E-25

0.1250

1.1884

0.0044

thymosin beta-12

Lateolabrax japonicus

P33248

80

2.00E-13

0.1250

1.1876

0.0003

similar to 19.9 kD myosin light chain isoform 1

Danio rerio

XP_685183

332

1.00E-89

0.1250

1.1720

0.0046

ictacalcin

Ictalurus punctatus

AAY86967

145

1.00E-33

0.1250

1.1607

0.0021

tropomyosin

Theragra chalcogramma

BAC44994

281

2.00E-74

0.1250

1.1368

0.0090

N-acylsphingosine amidohydrolase

Takifugu rubripes

AAM43813

367

e-100

0.1250

1.1196

0.0082

alanine-glyoxylate aminotransferase

Platichthys flesus

CAH59400

345

2.00E-93

0.1250

1.0860

0.0059

titin b

Danio rerio

ABG48499

65

5.00E-09

0.1250

-1.0841

0.0045

alpha-2,8-polysialyltransferase IV

Oncorhynchus mykiss

BAC77411

70

1.00E-10

0.1250

-1.0878

0.0030

hedgehog acyltransferase-like, a

Danio rerio

NP_957181

295

1.00E-78

0.1250

-1.1419

0.0074

parvalbumin

Cyprinus carpio

CAC83659

173

7.00E-42

0.1250

-1.1675

0.0033

BTEB transcription factor

Pimephales promelas

ABO28528

107

1.00E-21

0.0625

-1.2019

0.0625

*

myosin regulatory light chain 2

Salmo salar

CAD89610

330

7.00E-89

0.1250

-1.2121

0.0020

similar to Clca1 protein

Danio rerio

XP_694323

198

2.00E-49

0.0625

-1.2547

0.0625

*

ependymin

Perca flavescens

ABU49423

168

2.00E-40

0.0625

-1.2602

0.0625

*

aspartoacylase

Danio rerio

NP_001103573

384

e-105

0.0625

-1.3905

0.0625

*

    Immune

carboxypeptidase B

Paralichthys olivaceus

BAC53789

365

2.00E-99

0.1250

1.2897

0.0041

fish-egg lectin (FEL)

Cyprinus carpio

P68512

192

2.00E-47

0.1250

1.1769

0.0003

procathepsin B

Oncorhynchus mykiss

AAK69705

346

1.00E-93

0.0625

-1.1727

0.0625

*

gamma-glutamyl hydrolase

Danio rerio

NP_998487

223

6.00E-57

0.0625

-1.1939

0.0625

*

membrane glycoprotein

Human coronavirus

ABD75532

53

1.00E-05

0.0625

-1.1945

0.0625

*

beta-2 microglobulin

Salmo salar

AAG17525

176

8.00E-43

0.1250

-1.1995

0.0034

microtubule-associated protein 1 light chain 3 alpha

Danio rerio

NP_999904

238

3.00E-61

0.0625

-1.2245

0.0625

*

microtubule-associated protein, RP/EB family, member

Danio rerio

NP_998805

272

1.00E-71

0.0625

-1.2298

0.0625

*

T-cell receptor beta chain ANA 11, putative

Brugia malayi

EDP38115

63

2.00E-08

0.1250

-1.2600

0.0052

glycerophosphodiester phosphodiesterase domain containing 1

Danio rerio

NP_001004118

322

2E-86

0.0625

-1.2652

0.0625

*

CHK1 checkpoint homolog

Xenopus tropicalis

CAJ83813

92

2.00E-17

0.1250

-1.3981

0.0013

    Apoptosis

tissue inhibitor of metalloproteinase 2

Oncorhynchus mykiss

AAU14867

265

3.00E-69

0.1250

1.1235

0.0082

cathepsin H

Danio rerio

NP_997853

300

5.00E-80

0.1250

1.0898

0.0069

caspase-3

Dicentrarchus labrax

ABC70996

223

6.00E-68

0.1250

-1.0647

0.0022

caspase-1

Dicentrarchus labrax

ABB05054

79

3.00E-13

0.1250

-1.1270

0.0061

cathepsin S-like

Oncorhynchus mykiss

AAV32964

291

1.00E-77

0.1250

-1.1507

0.0047

    Redox and metal ion binding

hydroxymethylbilane synthase

Danio rerio

CAM15096

369

e-101

0.1250

1.3333

0.0042

hemopexin

Danio rerio

NP_001104617

313

2.00E-83

0.1250

1.2968

0.0052

transferrin

Salvelinus fontinalis

BAA84100

326

1.00E-87

0.1250

1.1769

0.0080

similar to leprecan-like 1 protein

Danio rerio

XP_695073

183

1.00E-44

0.1250

1.1543

0.0036

similar to synaptic glycoprotein SC2

Danio rerio

XP_693420

430

e-119

0.1250

-1.1340

0.0073

similar to LOC407663 protein

Danio rerio

XP_698537

124

6.00E-27

0.0625

-1.2429

0.0625

*

    Growth and development

yghl1 (Putative growth hormone like protein-1)

Seriola quinqueradiata

BAB62526

153

1.00E-35

0.1250

-1.1388

0.0072

ZPA domain containing protein

Oryzias latipes

NP_001098216

168

3.00E-40

0.0625

-1.1568

0.0625

*

    Detoxification

pregnane × receptor

Oncorhynchus mykiss

ABP38412

206

2.00E-51

0.0625

-1.2321

0.0625

*

    Osmotic stress

hyperosmotic glycine rich protein

Salmo salar

AAO32675

134

8.00E-30

0.1250

-1.1053

0.0021

    Digestion

similar to Apoa4 protein isoform 2 (Apolipoprotein)

Danio rerio

XP_698920

296

7.00E-79

0.1250

1.4959

0.0056

chymotrypsinogen 2-like protein

Sparus aurata

AAT45254

460

e-128

0.1250

1.4211

0.0095

pancreatic carboxypeptidase A1 precursor copy 2

Tetraodon nigroviridis

AAR16321

242

9.00E-63

0.1250

1.3204

0.0025

pancreatic protein with two somatomedin B domains

Paralichthys olivaceus

BAA88246

214

e-100

0.1250

1.1297

0.0021

chitinase (Zgc:55941)

Danio rerio

AAH44549

369

e-100

0.1250

-1.0618

0.0097


Genes selected for quantitative PCR analyses are shown in bold. * indicates genes responding at 0.0625 μg.L-1 esfenvalerate and ** indicates gene responding to both 0.0625 and 0.125 μg.L1 esfenvalerate. Remaining genes responded only at 0.125 μg.L1 esfenvalerate exposure. Information represents proportions of a limited number of available genes on the applied microarray.

Connon et al. BMC Genomics 2009 10:608   doi:10.1186/1471-2164-10-608

Open Data