Multiple protein alignment of defensin peptides. Most defensin peptides contain six canonical cysteine residues (A); Members of the CRS1C family contain eleven cysteines in a different spacing between each other; CRS4C-6 belongs to the CRS4C family but consists of ten instead of the usual nine cysteines for this group (B). A novel sequence (OTTMUSG00000019857) has been annotated within the defensin gene cluster region which lacks all the canonical cysteines in any known number and spacing. Four cysteine residues can be found here but they don't align with any of the known cysteines in other peptides (C). All cysteine residues are highlighted in yellow. Genes identified for the first time in this study are tagged as noveldef.
Amid et al. BMC Genomics 2009 10:606 doi:10.1186/1471-2164-10-606