Research article
Annotation and expression of carboxylesterases in the silkworm, Bombyx mori
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* Corresponding authors: Cheng Lu lucheng@swu.edu.cn - Ze Zhang zezhang@swu.edu.cn
1 The Institute of Agricultural and Life Sciences, Chongqing University, Chongqing 400044, China
2 The Key Sericultural Laboratory of the Agricultural Ministry of China, Southwest University, Chongqing, 400716, China
BMC Genomics 2009, 10:553 doi:10.1186/1471-2164-10-553
Published: 24 November 2009Additional files
Additional file 1:
Summary of the silkworm COE genes. NP indicates genes with no automatic prediction in silkworm. (N): missing N-terminal region; (C): missing C-terminal region. Chr.: chromosome. UN represents unknown chromosome locations.
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Additional file 2:
Sequence alignment of Lepidopteran AChEs. Three intrachain disulfide bridges are drawn between conserved Cys. The asterisks represent the catalytic triad (S200, E327 and H440).
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Additional file 3:
Chromosome distribution of all silkworm COEs. Arrows showed the transcriptional orientation.
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Additional file 4:
The phylogeny and expression patterns of BmCOEs. Bootstrap values > 70% are shown. The solid black circles corresponding to cDNA, EST, tissue microarray and RT-PCR column indicate that these genes have expression evidence. The genes with grey font are that they had no probes in microarray dataset. The underlined α-esterases mean that they had substitutions in the catalytic triad.
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Additional file 5:
Primers used in RT-PCR study. Primers used in RT-PCR study.
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