Additional file 8.

Validation of miRNA expression by qRT-PCR. a. Validation of selected miRNA genes by qRT-PCR using TaqMan miRNA Assays (left panel). qRT-PCR measurements were normalised to U6 small nuclear RNA (U6sRNA; bottom row). Expression data obtained by Luminex bead-based profiling (middle panel) showed good correlation with qRT-PCR data (right panel). b. Detailed description of TaqMan qPCR according to MIQE guidelines.

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Avril-Sassen et al. BMC Genomics 2009 10:548   doi:10.1186/1471-2164-10-548