BMC Genomics

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Open Access Highly Access Research article

Cell wall biogenesis of Arabidopsis thaliana elongating cells: transcriptomics complements proteomics

Elisabeth Jamet1*, David Roujol1, Hélène San-Clemente1, Muhammad Irshad1,3, Ludivine Soubigou-Taconnat2, Jean-Pierre Renou2 and Rafael Pont-Lezica1

Author Affiliations

1 Surfaces cellulaires et Signalisation chez les Végétaux, UMR 5546 CNRS - UPS -Université de Toulouse, Pôle de Biotechnologie Végétale, 24 chemin de Borde-Rouge, BP 42617 Auzeville, 31326 Castanet-Tolosan, France

2 UMR INRA 1165 - CNRS 8114 - UEVE, 2 rue Gaston Crémieux, CP 5708, 91057 Evry, France

3 Department of Botany, Govt. Post-Graduate College, 23200 Mardan, Pakistan

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BMC Genomics 2009, 10:505 doi:10.1186/1471-2164-10-505

Published: 31 October 2009

Additional files

Additional file 1:

Cell Wall Genes (CWGs) with detectable levels of transcripts in 5- or 11-day-old hypocotyls. The data provided represent the CWGs with their level of transcripts and statistical analysis.

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Open Data

Additional file 2:

Cell Wall Genes (CWGs) with high levels of transcripts in either 5- or 11-day-old hypocotyls. The data provided represent the CWGs having high levels of transcripts.

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Open Data

Additional file 3:

Secretory Pathway Genes (SPGs) with moderate or high levels of transcripts in 5- and 11-day-old etiolated hypocotyls. The data provided represent the levels of transcripts of SPGs and the corresponding statistical analyses.

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Open Data

Additional file 4:

Secretory Pathway Genes (SPGs) with modulated levels of transcripts in 5- and 11-day-old etiolated hypocotyls. The data provided represent the transcript levels of SPGs differentially expressed between the two samples.

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Open Data

Additional file 5:

Proteins extracted and identified by mass spectrometry from purified cell walls of 5- and 11-day-old etiolated hypocotyls of A. thaliana. The data provided represent the levels of transcripts of the genes encoding the proteins identified by proteomics.

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Open Data

Additional file 6:

RT-PCR analysis of the transcripts of some genes encoding CWPs identified through proteomics. The data provided represent the controls of microarray data by quantitative RT-PCR for some selected genes.

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Open Data

Additional file 7:

Nucleotide primers used for PCR amplifications. The sequences of the oligonucleotide primers used for PCR analysis are listed in this file.

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Open Data