Research article
EST and microarray analysis of horn development in Onthophagus beetles
-
* Corresponding author: Justen Andrews jandrew@bio.indiana.edu
1 Department of Biology, Indiana University, Bloomington, Indiana, 47405, USA
2 School of Informatics, Indiana University, Bloomington, Indiana, 47405, USA
3 Center for Genomics and Bioinformatics, Indiana University, Bloomington, Indiana, 47405, USA
BMC Genomics 2009, 10:504 doi:10.1186/1471-2164-10-504
Published: 30 October 2009Additional files
Additional file 1:
Summary of BLASTx results of Onthophagus taurus ESTs against UniProtKB. Summary of BLASTx sequence alignments of non-redundant sequences from Ot libraries (refer Results section) against UniProtKB protein dataset. The top BLASTx hits with E-values < 10-5 are shown.
Format: XLS Size: 317KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 2:
Summary of BLASTx results of Onthophagus taurus ESTs against FlyBase. Summary of BLASTx sequence alignments of non-redundant sequences from Ot libraries (refer Results section) against FlyBase protein dataset. The top BLASTx hits with the E-values < 10-5 are shown.
Format: XLS Size: 473KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 3:
Summary of BLASTx results of Onthophagus ESTs against all the datasets used in this study. Summary of BLASTx sequence alignments of Onthophagus ESTs against all the datasets used in this study. The bit score, E-value, and percent identity as well as the gene names from Tribolium, Drosophila, C. elegans, invertebrate, human, and nr dataset are shown for of each Ot non-redundant sequences.
Format: XLS Size: 1.4MB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 4:
List of genes exhibiting differential expression in horn- or leg primordia compared to abdominal epithelium. Listed are genes exhibiting significant (p < 0.05) and at least 2-fold difference in expression intensity in primordial head horns and/or prothoracic horns and/or legs. Shown are spot- or contig IDs, the tissue(s) in which differential expression was detected (H: head horns, T: prothoracic horns, L: legs), fold-differences in expression relative to abdominal epithelium (positive values indicate enrichment relative to abdominal epithelium), sequence match descriptions (UniProtKB, FlyBase, Triboium, invertebrate, or nr), percent amino acid sequence identity, E-values, and bit score.
Format: XLS Size: 83KB Download file
This file can be viewed with: Microsoft Excel Viewer