Table 1

Comparison of numbers of high confidence peptides and proteins detected by ProteinPilot based on the amino acid sequence database searched.

Sample

Database searched

Proteins detected

Distinct peptides detected


2005 mesocarp

MSDBa

1184

5679

2005 mesocarp

Custom Vitis DB with weightingb and trimmingc

1424

10793

2005 exocarp

MSDB

1390

6915

2005 exocarp

Custom Vitis DB with weighting and trimming

1493

12945

2005 exocarp

Custom Vitis DB with trimming but no weighting

1447

12956

2005 exocarp

Custom Vitis DB with weighting but no trimming

1382

8845


aRelease 20063108, Hammersmith Campus of Imperial College London

bEST clustering weighted higher scores for ESTs derived from Cabernet Sauvignon in order to avoid the potential loss of SNPs specific to this cultivar during consensus sequence determination by the PCAP software

cPredicted tryptic peptides that were determined to likely be truncated were trimmed and removed from the custom peptide database

Lücker et al. BMC Genomics 2009 10:50   doi:10.1186/1471-2164-10-50

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