Table 5

Bacterial strains and plasmids used in this study.

Strain or plasmid

Description

Reference


Strains


B. cenocepacia

K56-2

CF isolate, BCESM +,

[70]

K56-R2

cepR::Tn5-OT182 derivative of K56-2, TcR

[14]

K56-2ΔcepR

ΔcepR derivative of K56-2

This study

K56-2cciR

cciR::Tp derivative of K56-2, TpR

[16]

K56-2cciIR

ΔcciIR derivative of K56-2

[16]

K56-2cepRcciIR

cepR::Tp, ΔcciIR derivative of K56-2, TpR

[16]

E. coli

DH5α

F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG

Invitrogen

TOP10

F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG λ-

Invitrogen

SY327

araD, Δ(lac pro) argE(Am) recA56 rifRnalAλpir

[71]

Plasmids


pGPI-SceI

oriR6K, TpR, mob+, carries I-SceI cut site

[65]

pDAI-SceI

pDA17 carrying the I-SceI gene

[65]

TPCR2.1::H12

pCR2.1TOPO containing amplified homologous regions flanking cepR

This study

pGPI-SceI::H12

Mutageneis plasmid pGPI-SceI with homologous regions flanking cepR

This study

pAidA301

aidA::lux transcriptional fusion constructed in pMS402 KmR TpR

[28]

pAfcA

afcA::lux transcriptional fusion constructed in pMS402 KmR TpR

This study


O'Grady et al. BMC Genomics 2009 10:441   doi:10.1186/1471-2164-10-441

Open Data